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. 2008 Apr 21;154(5):1079–1093. doi: 10.1038/bjp.2008.142

Figure 7.

Figure 7

Effects of prostaglandin F2α (PGF2α) and bimatoprost on myosin light chain (MLC) phosphorylation. (a) HEK293/EBNA cells co-expressing FP and altFP4 receptors were pretreated with 10−6 M AGN211335 for 15 min (lanes 2 and 5) and with 10−7 M PGF2α for 30 min (lanes 2 and 3) or with 10−7 M bimatoprost for 30 min (lanes 5 and 6). Arrows indicate MLC phosphorylation. (a, right panel) The data represent mean±s.d. of three independent experiments. **P<0.01 versus control; ***P<0.01 versus bimatoprost alone. The intensities of the total MLC bands were used to normalize the protein-loading differences. (b) HEK293/EBNA cells expressing the FP receptor were pretreated with 10−6 M AGN211335 for 15 min (lanes 2 and 5) and with 10−7 M PGF2α for 30 min (lanes 2 and 3) or with 10−7 M bimatoprost for 30 min (lanes 5 and 6). Arrows indicate MLC phosphorylation. (b, right panel) The data represent mean±s.d. of three independent experiments. **P<0.01 versus control. The intensities of the total MLC bands were used to normalize the protein-loading differences.