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. 2008 Jun 16;155(1):84–92. doi: 10.1038/bjp.2008.232

Figure 1.

Figure 1

Effect of calcitriol on vitamin D receptor (VDR) and 25-hydroxyvitamin D-24-hydroxylase (CYP24A1) mRNA expression in human airway smooth muscle (ASM) cells. Cells were treated for 24 h with 100 nM of calcitriol. Total mRNA (2 μg) was then subjected to reverse transcription-PCR using VDR (top gel), CYP24A1 (middle gel) or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (lower gel) primers. PCR products were resolved on a 1% agarose gel and stained with ethidium bromide. Data are representative of three different cell lines.