Figure 5.

Effects of the combination of fluticasone with calcitriol on chemokine secretion in tumour necrosis factor alpha (TNFα)/interferon gamma (IFNγ)-treated airway smooth muscle (ASM) cells. Cells were pretreated with calcitriol (100 nM), fluticasone (Fp) (100 nM) or a combination of both agents for 2 h before incubation with TNFα (10 ng mL⁻¹) and IFNγ (500 IU mL⁻¹) for an additional 24 h. Secretion of RANTES (a), IP-10 (b) and fractalkine (FKN) (c) was analysed as described under Materials and methods. Values shown are mean±s.e.mean of three separate experiments. ^*P<0.05 compared with cells treated with TNFα and IFNγ; ^**P<0.01 compared with cells treated with TNFα and IFNγ.