Figure 4.

Effect of chemokines on ERK1/2, JNK1/2, p38, and CREB in hippocampal neuronal cultures. Western blots of total cell lysates (A) and of nuclear extracts (B) were obtained from pure neuronal cultures treated with 100 nM fractalkine after a 4-hr preincubation in balanced salt solution in the absence of glia. Antibodies selectively recognizing the activated form of ERK1/2, JNK1/2, p38, and CREB were used. Similar results were obtained with hMDC (10 nM; not shown). (C) Densitometric analysis of the effect of fractalkine (100 nM) on ERK1/2, JNK1/2, p38, and CREB. Each data point represents the average of two to three similar experiments. (D) Nuclear localization of activated CREB (pCREB) in neurons treated with 10 nM hMDC, 100 nM fractalkine, and 100 nM SDF-1α (10 min in the absence of glial feeder layer).