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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Cytokine. 2008 Mar 14;42(1):32–38. doi: 10.1016/j.cyto.2008.02.002

Figure 3.

Figure 3

ERK phosphorylation and protein expression in splenic T cells with anti-CD3 stimulation (anti-CD3 +) or without stimulation (anti-CD3 −) from sham animals treated with BSA (Sham+BSA), shams treated with E2-BSA (Sham+E-B), trauma-hemorrhage (T-H) treated BSA (T-H+BSA), TH treated with E2-BSA (T-H+E-B), T-H treated with E2 (T-H+E2), and T-H treated with E2-BSA and ICI 182 780 (T-H+E2+ICI). T cells were isolated 2 hrs after T-H and resuscitation, stimulated with 1 µg/mL anti-CD3 for 5 min, and lysed. Lysates were then analyzed for ERK phosphorylation (p-ERK) and protein expression (A). Blots were reprobed for β-actin for equal protein loading in various lanes. ERK blots obtained from 5 animals were analyzed using densitometry, and densitometric values for phosphorylation and total protein were normalized to β-actin and are shown as mean ± SEM in Panels B and C respectively. *p<0.05 compared to respective sham.