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. 2008 Jul 2;3(7):e2572. doi: 10.1371/journal.pone.0002572

Figure 1. Late passage, polyploid, Pim-1-expressing RWPE1 prostate cells are tumorigenic in nude mice.

Figure 1

(A) Western blotting for Pim-1 and actin in RWPE1 cells overexpressing Pim-1 or vector control (Neo). Early and late passage cells are shown. (B) Cell cycle profiles of Pim-1 overexpressing RWPE1 cells shows polyploidy in late passage cells. (C) FISH analysis of late passage Pim-1 overexpressing RWPE1 cells using probes for chromosomes 13 and 21 show chromosome doubling. (D) Sample images of H&E stained sections from RWPE1 xenografts. Only late passage Pim-1 expressing cells were tumorigenic.