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. 2008 Jul 2;3(7):e2572. doi: 10.1371/journal.pone.0002572

Figure 2. Isolation of matched diploid/polyploid RWPE1-Pim-1 cells by cell sorting.

Figure 2

(A) Diagram showing scheme for isolation of diploid (2N) and polyploid (>4N) Pim-1 cells by FACS sorting based on DNA content. Bottom panel is the FACS profile of sorted cells after several passages to get enough cells for FACS analysis. (B) Western blotting of Pim-1 and other markers in FACS sorted cells shows similar expression levels in sorted diploid and polyploid cells. (C) Cell counting of diploid and polyploid Pim-1 overexpressing cells. (D) Western blotting shows that the p53 pathway is intact in all FACS-sorted RWPE1 cells as demonstrated by the induction of p53 and p21 following daunorubicin treatment.