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. 2008 Jul 2;3(7):e2572. doi: 10.1371/journal.pone.0002572

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Roh et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Percentage of cells showing total chromosome number, numerical and structural aberrations from FACS-sorted RWPE1 cells. Total 31 to 40 metaphase spreads were analyzed per sample and scored for the chromosome number, numerical aberrations, and structural aberrations. (B) Representative SKY images of sorted Neo, diploid and polyploid RWPE1-Pim-1 cells. Neo: 49,X,del(Y),+5, t(9;20;X),t(11;18),+12,del(12),t(12;21),t(14;18),+15,t(14;18),+20,t(18;20). Diploid: 49,X,del(Y),t(3;4),+5,+5,t(9;20;9),+12,t(5;12)(12;21),t(14;18),t(7;18),+20,t(17;18;20). Polyploid: 97,XX,del(Y),+1,+1,+1,t(1;5),+2,+2,+3,+3,+3,t(3;7),+4,+4,+5,+5,+5,+5,+5,+5,t(5;21),+6,+6,+7,+8,+8,+9,+9,t(9;20;9)×2,+10,+10,+11,+11,+12,+12,+12,+12,t(5;12)×2(12;21)×2,+13,+13,+14,+14,t(14;18)×2,+15,+15,+16,+16,+17,+17,+18,+18,t(7;18)×2,+19,+19,+20,+20,+20,+20,t(17;18;20)×2,+21,+22. (C) Table showing structural chromosomal aberrations. The numbers of derivative chromosomes observed are shown. Polyploid-specific abnormalities are shown in bold.