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. 2008 Jul 2;3(7):e2572. doi: 10.1371/journal.pone.0002572

Figure 4. Polyploidy promotes the tumorigenicity of RWPE1 prostate cells in vitro and in vivo.

Figure 4

(A) Soft agar assay with colony counting in FACS-sorted RWPE1 cells. Colonies larger than 0.5 mm in diameter were counted. Results represent average of triplicate experiments. *p<0.05. (B) Histological analysis of grafts of diploid and polyploid RWPE1-Pim-1 cells recombined with rat UGM and grafted under the kidney capsule. Grafts from polyploid cells contained foci of carcinoma-in-situ with loss of the basal cell marker p63 and high rates of mitotic cells (p-HH3, phospho-histone H3) while diploid cells formed largely small benign looking glands that express p63 and low rates of mitosis (p-HH3). A human-specific Ku70 antibody was used to confirm the human origin of glands. All images were taken at the same magnification of 4×.