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. 2008 Jul 2;3(7):e2580. doi: 10.1371/journal.pone.0002580

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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To ensure that HIV inhibition was not due to abnormalities selected for during clonal selection of cell lines, HIV replication was assessed in the bulk cell lines that expressed the GBV-C NS5A 152–167 peptide, the scrambled peptide (152–167Scr), and the mutated peptides (S158A, S158G, S158E) and compared to the vector control cell line (VC) (panel A). The pattern of HIV inhibition did not change appreciably when infections were followed for up to 20 days (panel B). 1–414 = cells expressing the full-length GBV-C NS5A and FS = cells expressing the GBV-C NS5A frame shift control.