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. 2008 Jul 2;3(7):e2580. doi: 10.1371/journal.pone.0002580

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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The infectious titer of mumps virus was not inhibited in Jurkat cells expressing full-length GBV-C NS5A (1–414) or the 152–181 NS5A peptide compared to the vector control (VC) or parent Jurkat cells (A). Mumps virus replication was increased in Jurkat cells expressing full-length NS5A (*; p = 0.029). Similarly, panel B demonstrates that VSV-G pseudotyped retroviruses transduced all NS5A and control cell lines with similar efficiency as measured by relative luciferase activity in VSV-G pseudotype particles relative to the no-envelope control particles (p>0.05 for all compared to VC). 152–167 = cells expressing this NS5A 16mer peptide, Scr = cells expressing scrambled 16mer peptide, and S-A, S-G, S-E = cells expressing the S158A, S158G, and S158E substitutions.