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. 2008 Jul 2;3(7):e2580. doi: 10.1371/journal.pone.0002580

Figure 8. GBV-C NS5A synthetic peptides inhibit HIV replication.

Figure 8

Addition of a synthetic peptide representing GBV-C NS5A amino acids 152–191 (P1) to MT-2 cells 1 hour before HIV infection resulted in a dose-dependent inhibition of HIV replication (concentration of peptide = 10, 25, or 50 µg/ml; panel A). By comparison, overlapping peptides including GBV-C NS5A amino acids 172 to 211 (P2) or amino acids 197 to 236 (P3) did not inhibit HIV replication at 50 µg/mL. All HIV replication was compared to cells incubated with no peptide (NP). Addition of peptide 1 (amino acids 152–191) to Jurkat cells 24 hrs prior to HIV infection [(1) HIV] resulted in inhibition of HIV on day 4 († p<0.05; panel B). In cells in which peptide was not included after HIV infection (− P), HIV inhibition was lost on day 5. When HIV and the peptides were added to cells simultaneously (HIV+P), inhibition was not observed on day 4; however, when the peptide was included in the media (+P), HIV replication was inhibited by day 5 (* p<0.05 compared to the NP control).