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Internalization of A. fumigatus conidia by HNEC. HNEC were incubated with FITC-labeled A. fumigatus conidia for 4 h, washed to remove unbound conidia, and fixed. HNEC were incubated with a rabbit anti-conidia antiserum and then with Texas-red-conjugated anti-rabbit antibody to label non-phagocytosed conidia. (A) Overlay of green and red channels; (B) red channel (non-phagocytosed conidia); (C) green channel (total conidia). Bar = 2 μm.
