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Transient IR isotope-edited absorbance of LDH/NADH·oxamate ternary complex (13C2 minus 12C2) after laser T-jump to 40°C at 1624 cm−1 (upper trace) and 1607 cm−1 (lower trace). The sample concentrations were (0.6:0.6:0.6 mM, LDH/NADH/oxamate, respectively) in D2O. A single exponential fit overlays the 1624 cm−1 transient, whereas a biexponential fit is shown for the 1607 cm−1 transient. The temperature-dependent static isotope-edited IR spectra are shown in the inset.
