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Transient IR isotope-edited absorbance of LDH/NADH·oxamate ternary complex (13C1 minus 12C1) after laser T-jump to 40°C at 1589 and 1613 cm−1. Sample concentrations were (0.6:0.6:0.6 mM, LDH/NADH/oxamate, respectively) in D2O. A single exponential fit overlays the 1589 cm−1 transient and a biexponential fit overlays the 1613 cm−1 transient. The temperature-dependent static isotope-edited IR spectra are shown in the inset.
