TABLE 2.

Experimental dissociation constants, off-rate constants, and calculated association rate constants in 100 mM phosphate buffer

System	Kd (M)	koff (s−1)	kon (M−1s−1)
AFS-QDB	4.3 × 10−10	4.0 × 10−5	9.3 × 104
FSS-QDB	2.3 × 10−9	3.4 × 10−5	1.5 × 104
QDS-QDB	4.0 × 10−10	4.0 × 10−5	1.0 × 105
QDS-(40 nm) FSB	1.3 × 10−10	3.4 × 10−4	2.6 × 106
AFS-(40 nm) FSB	2.8 × 10−10	1.4 × 10−4	5.0 × 105
QDS-(200 nm) FSB	1.9 × 10−10	4.0 × 10−4	2.1 × 106
AFS-(200 nm) FSB	1.4 × 10−10	1.1 × 10−4	7.9 × 105