TABLE 3.
Calculated theoretical frequency factors (ASB) for native and labeled biotin-streptavidin binding systems in 100 mM phosphate buffer
| System | nsites (strept) | nsites (biotin) | frac-avail | ASB | Afree/ Alabeled |
|---|---|---|---|---|---|
| Native (2) | 4 | 1 | 0.16 | 1.67 | - |
| AFS-QDB | 3 | 6 | 2.5 × 10−4 | 1.2 × 10−3 | 1349 |
| FSS-QDB | 21 | 6 | 7.9 × 10−5 | 3.5 × 10−4 | 4812 |
| QDS-QDB | 10 | 6 | 8.4 × 10−4 | 4.1 × 10−3 | 405 |
| QDS-(40 nm) FSB | 10 | 148 | 9.3 × 10−4 | 5.7 × 10−3 | 293 |
| AFS-(40 nm) FSB | 3 | 148 | 8.6 × 10−4 | 7.6 × 10−3 | 220 |
| QDS-(200 nm) FSB | 10 | 18,540 | 7.8 × 10−3 | 1.3 × 10−1 | 13 |
| AFS-(200 nm) FSB | 3 | 18,540 | 7.2 × 10−2 | 2.0 × 10−1 | 8 |