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Experimental association rate constants and theoretical frequency factors for native and labeled systems in 100 mM phosphate buffer
| System | kon (M−1s−1) | kfree/klabeled (experimental) | Afree/Alabeled (theoretical) |
|---|---|---|---|
| Native (2) | 2.0 × 109 | - | - |
| AFS-QDB | 9.3 × 104 | 21,505 | 1349 |
| FSS-QDB | 1.5 × 104 | 135,294 | 4812 |
| QDS-QDB | 1.0 × 105 | 20,000 | 405 |
| QDS-(40 nm) FSB | 2.6 × 106 | 765 | 293 |
| AFS-(40 nm) FSB | 5.0 × 105 | 4000 | 220 |
| QDS-(200 nm)FSB | 2.1 × 106 | 950 | 13 |
| AFS-(200 nm)FSB | 7.9 × 105 | 2532 | 8 |
