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. Author manuscript; available in PMC: 2008 Jun 26.
Published in final edited form as: J Immunol. 2006 Jun 1;176(11):6802–6811. doi: 10.4049/jimmunol.176.11.6802

FIGURE 5.

FIGURE 5

The loss of MyD88 results in diminished MIP-2 and IL-12 p40 expression. Primary microglia from MyD88 KO and WT mice were seeded at 2 × 105 cells/well in 96-well plates and incubated overnight. The following day, cells were exposed to either 107 heat-inactivated S. aureus, 10 µg/ml PGN, or 100 ng/ml LPS for 24 h, whereupon conditioned supernatants were collected and analyzed for MIP-2 (A) and IL-12 p40 (B) protein expression by ELISA. Results are presented as the amount of cytokine (nanograms) per milliliter of culture supernatant (mean ± SD). Significant differences in cytokine expression between unstimulated vs PAMP-treated microglia are denoted with asterisks (*, p < 0.05; **, p < 0.001), whereas significant differences between MyD88 KO vs WT micro-glia are denoted with a hatched sign (##, p < 0.001). Results are representative of three independent experiments.