Figure 3. Microglia of DAP12^KI mice display a partially activated phenotype at birth.

(A) Quantitative analysis of the expression of inflammatory markers in DAP12^KI microglia. Quantitative RT-PCR performed on pure microglia cultured from brains of WT or DAP12^KI P0 pups. The levels in the mutant microglial cultures are expressed relative to the levels in the WT microglial cultures. n = 3 pairs of cultures. **p<0.05, Student's t test. (B) F4/80 labeling of microglia (arrows) in the hippocampus of WT and DAP12^KI pups. Scale bar: 100 µm. (C) Quantification of microglial density in the hippocampus of WT and DAP12^KI animals at P0. Between 4 and 15 pairs of matched slices were counted per experiment, n = 3 experiments; **p<0.001, two-way ANOVA. (D) Quantification of microglial density (Iba-1 positive cells) at P20 (n = 4 experiments).