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. Author manuscript; available in PMC: 2008 Jun 26.
Published in final edited form as: Biochemistry. 2006 Dec 19;45(50):15092–15099. doi: 10.1021/bi0613271

Figure 4.

Figure 4

HMGN1 inhibits the phosphorylation of nucleosomal H2A. (A) HMGN1 inhibits the Rsk2-mediated phosphorylation of nucleosomal H2A. Reaction mixtures containing equal amounts of core particles (CP), but various amounts of HMGN1, were incubated with [γ-32P] ATP and Rsk2, fractionated on 15% polyacrylamide SDS-containing gels. Shown are CBB-stained gels and corresponding autoradiograms. Note the dose-dependent, inverse correlation between HMGN1 input and H2A phosphorylation. (B) HMGN1 does not inhibit the Rsk2-mediated phosphorylation of purified H2A. Reactions as in panel A, except free, purified H2A was used as substrate. (C) Plot of the effect of HMGN1 on the Rsk2-mediated phophorylation of free and nucleosomal H2A. (D) HMGN1 inhibits the Msk1-mediated phosphorylation of nucleosomal H2A. The experiment has been done exactly as described in panel A, except for Msk1 as a kinase. (E) HMGN1 does not inhibit the Msk1-mediated phosphorylation of purified H2A. (F) Plot of the effect of HMGN1 on the Msk1-mediated phophorylation of free and nucleosomal H2A.