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. Author manuscript; available in PMC: 2008 Jun 26.
Published in final edited form as: Biochemistry. 2006 Dec 19;45(50):15092–15099. doi: 10.1021/bi0613271

Figure 5.

Figure 5

(A) HMGN1 inhibits the Rsk2-mediated phosphorylation of H2A by specific binding to nucleosome cores. Top: Mobility shift assays of purified chicken core particles (CP) with wild type HMGN1 and mutant the HMGN1S20,24 which does not bind to CP. Note that incubation of wild type HMGN1 (left) with CPs produced a distinct shift (CP+2HMGN1) while the HMGN1 S20,-24E mutant (right) produces a nonspecific smear. In phosphorylation assay HMGN1 S20,24E mutant does not inhibit the Rsk2-mediated phosphorylation of nucleosomal H2A (two bottom panels). (B) HMGN2 inhibits the phosphorylation of nucleosomal H2A. Shown are CBB-stained gels and corresponding autoradiograms. (C) The graph depicts the effects of HMGN1 (data from Figure 4), HMGN1 S20,24E, and HMGN2 proteins on the Rsk2-mediated phosphorylation of nucleosomal H2A.