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. 2008 Jul 2;3(7):e2556. doi: 10.1371/journal.pone.0002556

Figure 4. Bud emergence requires a functional exocyst.

Figure 4

(A) sec4-8, sec6-4 and isogenic wild type cells were grown 7 days in YPDA medium at 25°C. Cells were incubated with Con-A-FITC then washed with “old” YPDA and cells were shift to 37°C for 1 h. Cells were then re-fed with pre-warmed YPDA medium and grown either at 25°C or at 37°C as described in material and methods. The percentage of budded cells in wild type (blue), sec4-8 (purple) and sec6-4 (yellow) is indicated before and after exit from quiescence at 25°C or 37°C, N>200 for each time point, 2 experiments – error bars show SD. (B) Image of typical WT, sec6-4 and sec4-8 cells, 4 h after exiting quiescence at 25°C (left) and 37°C (right). (C) Localization of Sec8p-GFP before and after exit from quiescence at 30°C in the absence or in the presence of 200 µM Lat-A. Arrowheads point at Sec8p-GFP dots at the cell periphery. Bar 2 µm.