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. 2008 Apr;6(3):281–294. doi: 10.1111/j.1467-7652.2007.00314.x

Figure 1.

Figure 1

Expression of the Ashbya gossypii phosphoribosylpyrophosphate synthetase (PRS) gene in leaves of Arabidopsis thaliana and Nicotiana tabacum transformants. Plants were grown on selection medium, and resistant plantlets were transferred to soil and grown under standard cultivation conditions. The leaves of these plants were analysed 3–5 weeks after transfer for steady-state PRS mRNA levels using the full-length cDNA coding for PRS as hybridization probe. The same amounts of RNA were analysed. AtP, A. thaliana transformed with a construct to express the wild-type PRS gene of As. gossypii; NtP, N. tabacum transformed with a construct to express the wild-type PRS gene of As. gossypii; AtM, A. thaliana transformed with a construct to express a mutant form of the PRS gene of As. gossypii; NtM, N. tabacum transformed with a construct to express a mutant form of the PRS gene of As. gossypii. The numbers indicate the identity of the individual primary transformant. Asterisks indicate the lines that were further selected. C, control plants transformed with an empty vector.