Fig. 2.

Overexpression of RimJ alters ribosome profiles of the S5(G28D) strain. Sucrose gradient sedimentation analysis of ribosomes isolated from the S5(G28D) mutant strains with empty pBAD (pBAD) and RimJ overexpressed (p-rimJ). Positions of the 30S and 50S subunits and 70S ribosomes are indicated. Ribosome analysis of S5(G28D) strain with pBAD grown at 37°C (A), p-rimJ grown at 37°C (B), pBAD grown at 20°C (C), p-rimJ grown at 20°C (D). Ribosome analyses of the parental CSH142 strain grown at 37°C (E) or 20°C (F) are shown for comparison.