Table 1.
Calculated molecular mass (Arnold and Reilly, 1999) and that of the most prominent species of S5 determined by MALDI-TOF mass spectrometry.
| Protein | Sequence mass (Da) | Expected mass (Da; acetylated) | Experimental mass (Da) | Acetylation status |
|---|---|---|---|---|
| Wild-type S5 in CSH142 | 17472.3 | 17514.8 | 17515.0 | Acetylated |
| S5(G28D) in S5(G28D) strain | 17530.2 | 17572.7 | 17574.0 | Acetylated |
| Wild-type S5 in ΔrimJ | 17472.3 | 17514.8 | 17473.9 | Not acetylated |
| Wild-type S5 in ΔrimJ + pBAD | 17472.3 | 17514.8 | 17473.8 | Not acetylated |
| Wild-type S5 in ΔrimJ + p-rimJ | 17472.3 | 17514.8 | 17509.2 | Acetylated |
| Wild-type S5 in ΔrimJ + p-rimJ(C54A, C105A) | 17472.3 | 17514.8 | 17471.1 | Not acetylated |
| S7 in CSH142 | 19888.0 | NA | 19890.9 | Not acetylated |
R-protein S7 has been analysed for comparison.
NA, not applicable.