Figure 4.

Confirmation of selected gene expression by RT-PCR. (a) RT-PCR was performed for Oas1b, Fcre1g, Clu and Mif genes. Expression was normalized with housekeeping gene GAPDH. Lane 1: Negative control, Lane 2: uninfected saline control, Lane 3: 24 hr, Lane 4: 48 hr, Lane 5: 72 hr, Lane 6: 96 hr post infection. (b) Quantitative estimation was done by densitometry analysis of PCR product using Scion Image Analysis Software (Scion Corporation, Frederick, Maryland 21701). Values were normalized to the corresponding values of the house keeping genes for individual samples. Values are expressed as mean ± SEM. * p ≤ 0.05 compared to uninfected control.