Figure 3.

System-wide Effect of Replication Inhibitors on the Chromatin Proteome

Sperm nuclei were incubated in either untreated extracts or extracts supplemented with geminin or roscovitine.

(A and B) Chromatin proteins were identified and sorted into the 12 FCM clusters. For each protein, the cluster membership was compared between the untreated sample and in the presence of either geminin (A) or roscovitine (B). Column Rp presents reproducibility level. Proteins falling along the highlighted diagonal did not change cluster membership on addition of the inhibitor. Proteins that were present in the replicating chromatin but were not detected on chromatin after addition of inhibitors are recorded in the “lost” column.

(C) Effect of replication inhibitors on nuclear size. Error bars indicate the standard deviation (SD).