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. 2008 Apr 25;54(6):1083–1093. doi: 10.1111/j.1365-313X.2008.03481.x

Figure 3.

Figure 3

In vitro pull-down assays to test for interaction between various regions of LeETR1, NR and LeCTR2. (a) Western blotting to detect GST–receptor fusions using an anti-GST antibody. Lane 1, GST–ETR1F1–754; lane 2, GST–NR1–635; lane 3, GST–ETR1132–754; lane 4, GST–ETR1HKR364–754; lane 5, GST–ETR1HK364–647; lane 6, GST–ETR1R647–754; lane 7, GST control. mw, molecular weight markers. (b) Western blotting to detect DB–CTR2N50–700 (lane 1), DB–CTR2C680–982 (lane 2) and LexA control (lane 3) in yeast S. cerevisiae strain EGY48 using an anti-LexA antibody. mw, molecular weight markers. (c) Western blotting to detect DB–LeCTR2 fusions after incubation with GST–receptor fusions and pull-down using an anti-LexA antibody. 2N, DB–CTR2N50–700; 2C, DB–CTR2C680–982. The DB–CTR2N50–700 fusion protein was only detected (98 kDa, arrow) after incubation with GST–ETR1F1–754, GST–ETR1132–754, GST–ETR1HKR364–754 or GST–ETR1HK364–647.