Figure 1:

btn1Δ cells are temperature sensitive for growth at 37°C.A) Phase contrast of wild-type 972 and btn1Δ cells grown for 18 h at 37°C. B) Graph of number of cell cycles completed in 18 h in YES at 37°C, as determined by cell counting (three replicates). C) Growth curve of wild-type 972 compared with btn1Δ cells in 9 h following a temperature shift from 25°C to 37°C. Scale bar, 10 μm.