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. 2008 Jun;9(6):936–950. doi: 10.1111/j.1600-0854.2008.00735.x

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© 2008 The Authors Journal compilation © 2008 Blackwell Publishing Ltd

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Figure 4: — Polarization of sterol-rich domains, F-actin patch formation and polarization and endocytosis are linked.Loss of polarisation of sterol domains is linked to defects in F-actin patch formation/polarisation: Ai) filipin staining of wild-type cells treated with Latrunculin A (Lat A) for indicated times and ii) spatial distribution graph of filipin density of those cells that were in interphase; Bi) filipin staining of the actin ts mutant strain cps8-188 grown at 25°C and 37°C for indicated times and ii) spatial distribution graphs of filipin density of interphase and septated strains grown at 37°C for 4 h. Loss of polarisation of sterol domains is linked to defects in endocytosis: Ci) filipin staining of F-actin patch mutant myo1Δ and endocytic mutant end4Δ grown at 25°C and ii) spatial distribution graph of filipin density of indicated cells in interphase and septation, n = 25 individual measurements. Scale bar, 10 μm.