Figure 7:

Btn1p has an altered intracellular distribution at 37°C.A) Polarized location of GFP–Btn1p at 37°C: location of GFP–Btn1p in wild-type ED665 cells after overnight induction at 25°C followed by growth for 4 h at i) 37°C or ii) 25°C. B) Cell tip localization of GFP–Btn1p is actin dependent: wild-type ED665 cells expressing GFP–Btn1p at 4 h at 37°C treated with 10 μm Latrunculin A (Lat A) for 10 min at 37°C. C) GFP–Btn1p colocalizes with early but not late FM4-64-staining vesicles near cell tips at 37°C: 2 min FM4-64 uptake in wild-type ED665 cells expressing GFP–Btn1p at 4 h at 37°C (upper panel) and 20 min FM4-64 uptake followed by 40-min chase in wild-type ED665 cells expressing GFP–Btn1p grown at 37°C for 4 h (including labelling time) (lower panel). D) GFP–Btn1p localizes adjacent to, or partially overlapping, polarized F-actin patches: wild-type ED665 cells expressing GFP–Btn1p at 37°C for 4 h were fixed and stained for actin with rhodamine–phalloidin. Scale bar, 10 μm.