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. Author manuscript; available in PMC: 2008 Jun 26.
Published in final edited form as: Oncol Rep. 2006 Jan;15(1):275–281.

Figure 2.

Figure 2

Horehound leaves and wild cherry bark extracts suppress HCT-116 cell growth and induce apoptosis in human colorectal cancer cells. (A) HCT-116 cells were plated at 1,000 cells/well in 96-well plates and treated with vehicle, horehound (100 and 250 μg/ml) or wild cherry extracts (100 and 250 μg/ml) in the presence of serum. After 48 h, cell growth was measured using the CellTiter 96 AQueous One Solution Cell Proliferation Assay. Values are expressed as mean ± SD of 6 replicates. Statistical significance is according to Student’s t-test. *P<0.05, ***P<0.001 versus vehicle-treated cells. (B) HCT-116 cells were plated at 3×105 cells/well in 6-well plates and treated with vehicle, horehound (100 and 250 μg/ml) or wild cherry extracts (100 and 250 μg/ml) in the presence of serum. After 24 h, the cells were stained with Annexin V and PI and analyzed by flow cytometry as described in Materials and methods. Values are expressed as mean of 2 replicates.