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. Author manuscript; available in PMC: 2008 Jun 26.
Published in final edited form as: Oncol Rep. 2006 Jan;15(1):275–281.

Figure 4.

Figure 4

NAG-1 promoter activity on horehound leaves and wild cherry bark extracts treatment. pNAG−966/+41 (0.5 μg) was transiently transfected with 0.05 μg of pRL-null vector into HCT-116 cells. Subsequently, the cells were treated with vehicle, horehound (10 and 100 μg/ml) or wild cherry extracts (10 and 100 μg/ml) in the absence of serum for 24 h. The promoter activities were measured by luciferase activity. Transfection efficiency for luciferase activity was normalized to the Renilla luciferase (pRL-null vector) activity. The y axis shows fold increase of relative luciferase unit (RLU) compared to RLU of vehicle-treated cells. The date represents mean ± SD from three independent experiments. Statistical significance is according to Student’s t-test. *P<0.05, ***P<0.001 versus vehicle-treated cells.