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. Author manuscript; available in PMC: 2008 Jun 26.
Published in final edited form as: Oncol Rep. 2006 Jan;15(1):275–281.

Figure 5.

Figure 5

Wild cherry bark extract suppresses β-catenin signaling in SW480 cells. (A) SW480 cells were treated with vehicle, horehound (10 and 100 μg/ml) or wild cherry extract (10 and 100 μg/ml) in the absence of serum for 24 h. The cell lysates were harvested to perform Western blot analysis. (B) TOPFLASH or FOPFLASH β-catenin reporter construct (0.5 μg) was transiently transfected with 0.05 μg of pRL-null vector into HCT-116 cells, and then the cells were treated with vehicle, horehound (10 and 100 μg/ml) or wild cherry extract (10 and 100 μg/ml) in the absence of serum for 24 h. Transfection efficiency for luciferase activity was normalized to the Renilla luciferase activity. Data were expressed as the ratio of TOPFLASH to FOPFLASH. Each value represents mean ± SD from three independent transfections. The value obtained from vehicle-treated cells was defined as 100%. Statistical significance is according to Student’s t-test. **P<0.01 versus vehicle-treated cells.