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Purinergic receptor profile and signaling in cultured GENCs. A–B: RT-PCR detection of purinergic receptor mRNA in wild-type (A) and Cx40 siRNA-treated GENCs (B). No changes in receptor expression were found in response to siRNA knockdown of Cx40. C: representative recordings of 100 μM exogenous ATP-induced elevations in [Ca2+]i in wild-type (WT) and Cx40 siRNA-silenced GENCs (Cx40 siRNA). Arrow indicates the time ATP was added to the bathing solution. Responses were nearly identical.
