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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Chem Res Toxicol. 2008 Feb 6;21(3):668–677. doi: 10.1021/tx7003695

Figure 4.

Figure 4

Oxidation of 11S,12S- and B[g]C-11R,12R-dihydrodiols by AKR1C9 alanine-scanning mutants. Alanine-scanning mutagenesis was performed for the contact residues in the AKR1C9 active site. With each mutant, the oxidation of (+)-11S,12S- and (-)-11R,12R-isomers of B[g]C-11,12-dihydrodiol (20 μM) was monitored spectrophotometrically over time with 2.3 mM NADP+ in 50 mM AMPSO at pH 9.0 at 37 °C. Initial velocities were determined by taking tangents to the progress curve and converted into kcat/Km using the relationship v/[S] ) Vmax/Km when [S] , Km. Nine replicates were performed in each case, and the standard error was <10% of the mean value.