FIGURE 6.

Inhibition of Cdk5 reduces CRMP2 phosphorylation at Ser-522 in intact cells. A, primary rat cortical neurons were treated with 10 μm purvalanol for the times indicated. Cell lysates were subjected to Western blot analysis using Ser(P)-522 (upper panel), Thr(P)-514/509 (middle panel), and total CRMP2 (lower panel) antibodies. B, same as A, except SH-SY5Y cells were treated with purvalanol for the times indicated. C, same as B, except the SH-SY5Y cells were treated with 2 μm CT99021. D, same as B, except SH-SY5Y cells were treated with 10 μm alsterpaullone. In all cases representative blots of two experiments are shown.