FIGURE 8.

Pin1 does not regulate Ser-522 phosphorylation. A, HEK293 cells were co-transfected with FLAG-CRMP2 and wild type (WT) or mutant Pin1 as indicated. After 24 h, CRMP2 was isolated from lysates using anti-FLAG-agarose and subjected to Western blot analysis, and membranes were probed with Thr(P)-514/509 (upper panel), Ser(P)-522 (middle panel), or total CRMP2 (lower panel) antibodies. B, lysates from wild type, Pin1-knock-out (KO), and Pin1-OE (over-expressor) cortex were subjected to Western blot analysis, and membranes were probed as in A.