FIGURE 6.

Effect of PPAR agonists or antagonist on SDC-1 expression in PC-3 cells. A, cells were treated with 100 μg/ml n-3 LDL or 5 and 10 μm PPARγ agonist, troglitazone, or 5 and 10 μm PPARδ agonist, GW610742 for 24 h; B, cells were transfected with d/n-PPARγ cDNA and treated with 100 μg/ml n-3 LDL, 30 μm DHA, and 10 μm troglitazone for 8 h. C, cells were treated with 100 μg/ml n-3 LDL in the presence or absence of 15 μm PPARγ antagonist GW259662 for 8 h. Total RNA was isolated, and SDC-1mRNA was determined by real-time RT-PCR. Data are means ± S.E. in triplicate experiments. Values with different lowercase letters are significantly different (p < 0.05).