FIGURE 2.

Physical association of αTTP with lipid vesicles. A, purified recombinant αTTP (or glutathione S-transferase (GST); 1.5 μm) was incubated with the indicated concentration of sonicated unilamellar vesicles (SUV) for 30 min. Free protein was separated from membrane-bound protein by centrifugal filtration as described under “Experimental Procedures.” Shown is a representative of three independent experiments. B, fluorescence emission spectrum of αTTP (0.8 μm; excitation = 295 nm) was monitored in the presence or absence of 15 mm concentrations of sonicated vesicles. Fluorescence intensities were normalized at the wavelength of maximal emission and corrected for scattering. These results are representative of three experiments.