FIGURE 7.

Single channel activity of Rv1698_His purified from M. bovis BCG. Rv1698_His protein was purified by Ni²⁺ affinity chromatography from 0.5% OPOE extracts obtained from M. bovis BCG/pML911. Purified Rv1698_His protein was added to the cis-side of a DPhPC membrane bathed by 1 m KCl, 10 mm HEPES, pH 7.0, and a –10 mV potential was applied. The channel activity was recorded using a data acquisition card. The boxed traces highlight opening and closing events of different sizes. A, the current trace shows more than 30 opening and closing events recorded 600 s after addition of 300 ng of Rv1698_His to the membrane. The total recording time was 1843 s. B, the histogram represents a total of 109 opening and closing events recorded from seven membranes. C, the current trace shows more than eight opening and closing events recorded 400 s after the addition of 1μg of Rv1698_His to the membrane. The total recording time was 2862 s.