FIGURE 4.
Induction of HO-1 in response to Mtb infection is independent of the NO signaling pathway. A, increase in HO-1 mRNA in response to Mtb infection. RAW cells and BMM from iNOS2-/- mice were infected with Mtb, and HO-1 expression was analyzed using Q-PCR. Results are expressed as mean ± S.D. (n = 3 in triplicate). B, increase in HO-1 protein levels in response to Mtb infection. HO-1 protein is increased in RAW cells 24 h post infection and in the presence and absence of 1400W. Note that HO-1 can migrate as two bands (see “Results” for details).