FIGURE 6.

HO-1 is significantly induced in the lungs of Mtb-infected mice. A, relative HO-1 mRNA abundance measured in the lungs of uninfected (U) or Mtb-infected (I) (4 weeks post infection) C3HeB/FeJ mice. Results are expressed as mean ± S.D. (n = 4 in triplicate). B, immunohistochemistry of Mtb-infected lungs (4 weeks post infection). Expression of HO-1 protein within TB lung lesions was demonstrated using HO-1-specific antibodies. Panel I, staining with HO-1-specific antibodies; panel II, hematoxylin and eosin staining. TB lung lesions of C3HeB/FeJ mice (panels I and II) contained numerous HO-1-positive cells (brown color in panel I and inset) within cell wall surrounding areas of necrosis.