Figure 2.
Western blot analysis of PfCRT stage dependent expression, using a previously described specific polyclonal antibody [14]. Parasites were synchronized as described, and harvested at 0 hr (early rings) or hr 13 (late R / early T cycle n), 25 (late T), 31 (early S), 37 (segmented S), 48 (early R cycle n+1), 55 (mid - late R cycle n+1); Lanes 1 – 7, respectively, in the representative Western blot at the top. 10 µg parasite protein was added to each lane, and Western analysis for PfCRT was performed as described previously [14]. Five such analyses were done, the bands quantified by densitometry, and the averages are plotted for both Dd2 (open symbols) and HB3 (closed) parasites.