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. Author manuscript; available in PMC: 2008 Jun 26.
Published in final edited form as: J Biol Chem. 2007 Jan 2;282(9):6161–6171. doi: 10.1074/jbc.M607661200

Fig. 1.

Fig. 1

The role of Lat1 in CR. A, 0.5% glucose induced CR requires Lat1 to extend replicative life span. Life span analysis of the wild type cells and the lat1Δ mutant cells grown in 2% and 0.5% glucose. B, Lat1 over-expression extends replicative life span. Life span analysis of the wild type cells carrying a control vector, the Lat1 over-expression plasmid, the Lpd1 over-expression plasmid, the Pda1 over-expression plasmid and the pkp1Δ mutant. C, Lat1 over-expression does not synergize with 0.5% glucose to extend replicative life span. Life span analysis of the wild type and Lat1-oe cells grown under 2% and 0.5% glucose. D, 0.05% glucose induced CR requires Lat1 to extend replicative life span. Life span analysis of the wild type cells and the lat1Δ mutant cells grown in 2% and 0.05% glucose. E, Lat1 over-expression does not synergize with 0.05% glucose to extend replicative life span. Life span analysis of the wild type and Lat1-oe cells grown under 2% and 0.05% glucose. F, Northern blot analysis of the Lat1 mRNA levels in cells grown in 2%, 0.5% and 0.05% glucose CR conditions. WT: BY4742 wild type; Lat1-oe: Lat1 over-expression. One set of representative data is shown.