Skip to main content
. Author manuscript; available in PMC: 2008 Jun 26.
Published in final edited form as: J Biol Chem. 2006;281(6):3458–3462. doi: 10.1074/jbc.M512148200

FIGURE 4. The effects of mutations in the interdomain region.

FIGURE 4

A, top view of the visual arrestin crystal structure highlighting residues between the two domains that were mutated. B, the binding of each radiolabeled arrestin produced in cell-free translation to dark P-Rh (left), P-Rh* (center), and Rh* (right) is shown. Results are color-coded as follows: blue, mutations that significantly reduce P-Rh* binding; yellow, mutations that enhance dark P-Rh binding; green, mutations that enhance Rh* binding; gray, mutations that do not significantly affect binding. Means ± S.D. from two experiments performed in duplicate are shown. *, p < 0.05, **, p < 0.01, as compared with wild-type (WT) arrestin.