Table 1.
Plasmids and strains
| Plasmid or strain | Relevant phenotype or genotype | Reference or source |
|---|---|---|
| Plasmids | ||
| pUC19 | ApR | Messing (1979) |
| pSUP202 | ApR, TcR, CmR; Mob+; pBR322 derivative | Simon et al. (1983) |
| Litmus 28i | Multi-purpose cloning vector | New England BioLabs |
| pLA2917 | KnR, TcR; RK2 derivative, cos | Allen & Hanson (1985) |
| p34S-Tp | Source of dhfRII cassette; TpR | Dennis & Zylstra (1998) |
| pWT-P1 | 6.7 kb gfa-adhI-cycI-xoxF-cycB-xoxJ-rsp2581 PstI fragment cloned into pUC19 | Witthuhn, This study |
| pXoxF2 | 1958 bp EcoRI fragment containing xoxF | This study |
| pTP16 | 589 bp deletion and TpR insertion at xoxF codon 260 | This study |
| pTP16-12 | 1.9 kb EcoRI fragment containing ΔxoxF : : Tp from pTP16 cloned into pSUP202 | This study |
| pSW502 | 1.2 kb PstI–EcoRI fragment containing cycB | This study |
| pSW517 | 120 bp deletion and TpR insertion at cycB codon 71 | This study |
| pSW521 | 1.7 kb PstI–EcoRI fragment containing ΔcycB : : Tp from pSW517 cloned into pSUP202 | This study |
| pSW161 | 1.2 kb EcoRI fragment containing gfa | This study |
| pSW161-4 | 147 bp deletion and TpR insertion at gfa codon 74 | This study |
| pSW170 | 1.1 kb EcoRI fragment containing Δgfa : : Tp from pSW161-4 cloned into pSUP202 | This study |
| pUI8747 | TcR; gfa-adhI-cycI-xoxF-cycB-xoxJ DNA in pLA2917 | Rott et al. (1993) |
| Strains | ||
| R. sphaeroides | ||
| Ga | crtD | Laboratory strain |
| BOX9 | crtD ΔadhI : : V SpR box-9 : : Tn5TpR | Ozcan (1996) |
| CCMA1 | ccmA1 : : V | Cox et al. (2001) |
| CYCI1 | cycI : : V | Rott et al. (1993) |
| CYCAI | cycA cycI : : V | Rott et al. (1993) |
| TP39 | crtD cycA spd-7 Tn5 : : I, 2300 | Rott et al. (1993) |
| BC1 | fbcBC : : V TpR | Mouncey et al. (2000) |
| TP19 | crtD ΔxoxF : : Tp | This study |
| SW426 | crtD TP19 with pUI8747 | This study |
| SW531 | crtD ΔcycB : : Tp | This study |
| SW538 | crtD SW531 with pUI8747 | This study |
| SW102 | crtD Δgfa : : Tp | This study |
| E. coli | ||
| DH5α | supE44 ΔlacU169 (Φ80 lacZΔM15) hsdR178 recA1 endA1 gyrA96 thi-1 relA1 | Bethesda Laboratories Research (1986) |
| S17-1 | C600 : : RP-4 2-(Tc : : Mu) (Kn : : Tn7) thi pro hsdR hsdM+ recA | Simon et al. (1983) |