Figure 2.
Effect of Base Stacking on 8OHdG Detection Sensitivity. (A) 20 s injection of 8OHdG standard in Ringer's solution, (B) 20 s injection of 8OHdG standard in Ringer's solution followed by a 45 s injection of 0.1 M NaOH. Conditions: 100 mM ammonium hydroxide BGE with 0.5 mM TTAB at pH 9.5, 50 µm i.d. capillary × 80 cm, separation and injection at −15 kV, detection at + 600 mV vs. Ag/AgCl.