. Author manuscript; available in PMC: 2008 Jun 26.

Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Jul 1;827(1):16–25. doi: 10.1016/j.jchromb.2005.05.036

Table 1.

Optimization of Base-Stacking Parameters for 8OHdG in Ringer's Solution

		Sample in Ringer’s Solution, with Base-Stacking			Sample in BGE, without Base-Stacking

BGE	pH	Min. NaOH (s)	Peak Height (mAU)	Efficiency (N/1000)	Peak Height (mAU)	Efficiency (N/1000)
Methylamine	10.5	15	2.28 ± 0.11	231 ± 5	0.477 ± 0.006	45 ± 5
NH₄OH	9.2	15	1.69 ± 0.05	173 ± 6	0.530 ± 0.014	70 ± 5
Tris	8.0	5	2.05 ± 0.02	166 ± 16	0.435 ± 0.025	23 ± 7
Imidazole	7.0	8	9.39 ± 0.27	4,498 ± 15	0.542 ± 0.011	66 ± 6

Conditions: 30 s injection of 50 µM 8-OHdG standard in 90 % Ringer's for base-stacking or 10 s injection in BGE for normal electrokinetic injection, 100 mM BGE (pH ≈ pKa) with 0.5 mM TTAB, n=3, UV detection